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Image Search Results
Journal: Communications Biology
Article Title: σ E of Streptomyces coelicolor can function both as a direct activator or repressor of transcription
doi: 10.1038/s42003-023-05716-y
Figure Lengend Snippet: Western blot results show cellular levels of σ E in S. coelicolor LK3802 during 1 h incubation at 30 °C (or at 42 °C in the case of heat stress) under osmotic (0. 5 M NaCl), oxidative (0.5 mM Diamide), or EtOH (4%) stress. The σ E signal was normalized to the signal of the RNAP core (ß subunit) and the first time point (0 min) was set as 1. Representative primary data of σ E and RNAP signals are shown below the chart. The experiment was performed in two biological replicates. The bars are averages and the dots represent individual replicas.
Article Snippet: The RNAP core cellular level was detected using
Techniques: Western Blot, Incubation
Journal: Communications Biology
Article Title: σ E of Streptomyces coelicolor can function both as a direct activator or repressor of transcription
doi: 10.1038/s42003-023-05716-y
Figure Lengend Snippet: σ E binds to RNAP to form holoenzyme and binds to promoters where three possible scenarios can occur (i) σ E is the only regulator of the gene; (ii) σ E is one of two or more regulators; (iii) σ E is bound to the promoter, either occluding and overlapping another promoter or forming a barrier against RNAP that has initiated transcription from upstream promoters. σ E -dependent transcription can possibly be also modulated by additional binding partners such as ApgA, CarD, BldC, and DNA binding proteins NdgR, Lsr2.
Article Snippet: The RNAP core cellular level was detected using
Techniques: Binding Assay, DNA Binding Assay